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317 Serum albumin levels decrease with age, 318 possibly increasing A deposition in the brain, as albumin is able to sequester A from the blood

While proper loading time is relatively easily to establish and loading temperature is mostly the temperature used for cell culture (for example 37 C and 39 C for human and mouse cells and cell lines, respectively), additional fine-tuning is needed to establish proper loading concentration of the fluorescent dyes to avoid mitochondrial toxicity, non-specific binding to other cellular structures, photodamage and dye aggregate artifacts

doi: 10.1016/j.redox.2023.102643 76 HenrotinYKurzBAignerT

This leads to an overabundance of calcium ion (Ca 2 ) influx and subsequent excitotoxic neuronal injury, contributing to ototoxicity